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These rates were similar to those reported by Cohen et al. [ 21] based on an analysis of 52 patients diagnosed with small cell neuroendocrine carcinoma (SmCC) in Japan.
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After washing, the other parts of amino groups were reacted with sulfo-SMCC in PBS-EDTA buffer to introduce maleimide groups on the particle surface.
Thus, when the T7 data set as given in Figure 1 is analysed by program Clique, package Phylip version 3.64 [ 9] the only topological differences of the output tree in relation to the SMCC tree in Figure 9 are placements of R and K according to the unjustified assumption a) above at separate branches with zero character state changes for each of them.
SMcC assisted in study design and interpretation of findings.
SMcC participated in data collection, performed analysis on sample data and revised drafts of the manuscript.
SMcC participated in the interpretation of the results and drafted the manuscript.
After the unreacted APTES was removed by centrifugation, the NCs were mixed with 2 mM of SMCC dissolved in DMSO and kept at room temperature for 1 h.
Specifically, 100 μL of 14.35 μM Sulfo-SMCC solution in DMSO was added to 5 mg of nanoparticles in PBS-EDTA buffer under gentle shaking for 1 h at room temperature.
Peptides were conjugated to aminated microwell surfaces through the specific reaction between C-terminal cysteines and the maleimide-activated surfaces, as shown in Figure 1a. 10 mM SMCC was prepared in 1× PBS buffer, pH 7.4.
To further boost the learning performance, we extend our proposed GM-SMCC method in an ensemble manner by constructing multiple latent networks.
Of the 54 patients with available data, 25 (46%) had recognised predisposing medical conditions for the development of non-SmCC tumours in the same organ.
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