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Smashed samples were added into sterile 50 mL conical Falcon tubes and then extracted with acidified ethanol pH 3.5.
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After 15 minutes of boiling, the samples were smashed with glass beads (Mini-Beadbeater, Merlin Diagnostic Systems, Breda, The Netherlands) for two minutes.
Moreover, the limitation for amplifying ITS2 gene using this PCR demonstrated that 1×10−2 microfilaria of each species of parasite smashed or even with mixed samples could be detected and the PCR products were predicted as the same as that described above.
Words were exchanged with officials and Higgins smashed one of the two full sample bottles against a wall.
Samples were mounted in 70% glycerol, smashed using the cover slip and viewed with a Nomarski objective or phase contrast using the Leica DM IRB light microscope.
Playing from his 2014 studio album You're Dead!, Ellison showcased his experimental electro, smashing breakbeats and fizzing samples.
Toxe Gothenburg producer Tove Agélii (18) makes hype songs for dystopian pep rallies, littered with samples of smashing glass, industrial clatter and, on Xic, Britney Spears's gasps from Toxic.
Leaf samples of 100 mg were collected from the Tm and mvp mutant lines and smashed by TissueLyser (Qiagen) (shaking settings: 25 Hz, 1 min and 30 sec).
All samples were dried in a vacuum oven at 100 °C until constant weight and they were smashed into particles with size between 100 and 200 μm.
I swung, I smashed.
That was cruelly smashed.
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