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A steam generator tube rupture (SGTR) accident analysis for SMART was performed using the TASS/SMR-S code.
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BLAST (EMBL, Flybase) and domain searches (SMART) were performed to identify Drosophila proteins that contain the mucin-associated SEA, vWD4, EGF and cystein-knot domains, and identified proteins were manually scanned for the presence of PTS repeats.
A conservative analysis against a SMART SGTR was performed.
Furthermore, a domain profile search using Interpro and SMART [17] was performed to verify the domain structure of individual NLRs.
Introduction of Accell SMART pool was performed according to the manufacturer's instruction.
The "smart prep" scanning was performed in order to obtain a four chamber projection.
Transfection of a pool of four siRNA oligonucleotides specifically targeting p21 or BIM mRNAs (Smart Pool siRNA, Dharmacon) was performed with Hiperfect (Qiagen, Hilden, Germany).
Anonymous SMART scanning (RAW-format) was performed with activation of all analytical modules.
PCR was performed using Smart Cycler and data were analyzed using Smart Cycler System Software (Cepheid, Sunnyvale, CA).
To obtain the full-length cDNA, rapid amplification of cDNA ends (RACE) with nested Elovl5-specific primers and the SMART 3' and 5' oligonucleotide primers (SMART RACE cDNA Amplification Kit, Clontech) was performed, using TaKaRa polymerase (TaKaRa LA Taq Hot Start Version, Takara Bio Inc., Clonetech).
Raman spectroscopy was performed by Smart Raman DXR (Thermo Scientific) spectrometer.
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