We used SMART template-switching protocol and primer extension PCR [ 31], normalization and subtraction [ 32] as described [ 11].
We constructed cDNA libraries enriched for full-length inserts by applying the SMART template-switching protocol, primer extension PCR [ 20], normalization, and subtraction [ 21], as described by Federov et al. [ 22].
The first full-length cDNA library from liver (UAhib) was constructed without SMART template-switching and PCR amplification as described [ 39], and inserts were cloned into vector pCMV-SPORT6 (Invitrogen).
A "smarter" template with alerts based on glucose levels and nutritional status and forced rather than optional use, coupled with more intensive implementation support, might further improve the care of hospitalized patients with type 2 diabetes.
In this study, we showed that the control of such properties can be defined from the bottom-up, using smart surface templates to modulate the structure, composition, and bio-mechanics of human transplantable tissues.
In addition to explicit SIMD instructions and smart expression templates, the framework introduces domain specific constructs for the tensor cross product and its associated algebra recently rediscovered by Bonet et al.
Both the smart "self-template" effect and appropriate activation effect are crucial for the rational hierarchical porous structure.
His spiel is one I already recognize as fitting the smart-house template: Tell me what you want and I will make all the pieces of the puzzle fit.
