In the improved model, the distributed stiffness and mass of the embedded segment of the Smart Probe are explicitly formulated in the governing equations of motion, allowing more realistic predictions of the actual dynamic features of the Smart Probe-cementitious material system.
Finally, 'smart' probes are agents designed to activate exclusively in the presence of their intended target.
"Smart" or "activatable" MR probes are particularly attractive because they modulate their MR properties (relaxivity) leading to signal amplification upon molecule target interaction.
SMART-cDNA probes were evaluated for their temporal specificity using quantitative real-time PCR (qPCR) to amplify the transcripts of stage-specific genes (data not shown).
Moreover, the smart probes could be used to determine the kinetics of dNTP misincorporation and single nucleotide extension by proper design of the sequence.
These findings proved that smart (enzyme-activated) probes were applicable in NIFR imaging to monitor the efficacy of drugs.
The Cy5.5 fluorescence dye is linked to a specific substrate of matrix metalloproteinase to form a smart probe that is activated by the enzyme.
Transmission electron microscopy revealed that the multimodal SMART cancer probe was spheric and well dispersed.
A common strategy for enzyme-activated smart probe design is to engineer probes that produce signal when cleaved by a protease.
Fluorescent-labelled cDNA probes were synthesised using the BD Atlas™ SMART™ Fluorescent Probe Amplification Kit (BD Biosciences Clontech, UK).
The probes were labeled with isotope P. RACE was performed using SMART™ RACE cDNA synthesis kit (Clontech Inc., USA) according to the manufacture's instruction.
