For the targeting of Kif3A, a smart pool from Dharmacon (ON-target plus SMART pool L-004964-00-0005) was transfollowingllowing the same protocol.
The NAV3 gene was silenced with pooled siRNA oligonucleotides (On-Target SMART pool, Dharmacon, Chicago, IL, USA) as instructed by the producer.
siRNAs employed in this study were a part of a commercially available siGENOME SMART pool (Dharmacon).
The siRNAs for SKP1 were from Dharmacon's SMART pool.
Human HIF1-α smart pool siRNA was obtained from Dharmacon, (Lafayette, CO).
siGENOME SMART pool p300 and CBP siRNA molecules were purchased from Dharmacon.
RNA interference was performed with a "Smart Pool" mixture targeting human TBCD (Dharmacon, CO, USA).
Control siRNA and the Smart pool siRNA targeting p97 were purchased from Dharmacon (Lafayette, CO).
To knock down FASN, SiGenome ON-TARGET plus SMART pool duplex of FASN were purchased from DHARMACON (Cat# L-040091).
siGenome SMART pool siRNA targeting nucleolin or histone genes and negative scrambled siRNA control were obtained from Dharmacon.
The sequences of control siRNA and on-target smart pool siRNA for timeless were reported in Dharmacon.
