Exact(3)
The monk showed me to the newly refurbished men's guest house where the small, smart library included books by Fr.
The SMART library provided a useful resource for the functional genomic study of Ujumqin sheep and could present some new molecular material for this species as well.
rRNA depletion using the Ribo-Zero kit was efficient: the proportion of rRNA reads was less than 1% for the TS and OV libraries, and less than 3% for the SMART library (Table 2).
Similar(57)
This review presents computational methods that experimentalists can readily use to create smart libraries for enzyme engineering and to obtain insights into protein substrate complexes.
Challenges in genetic diversity creation were discussed with emphases on creating "smart" libraries, controlling the mutagenesis spectrum and specific challenges in each group of mutagenesis methods.
This approach is compatible with a recent trend in protein engineering that stresses construction of small and smart libraries.
By contrast, TS and SMART libraries appear to produce more accurate results, which were corroborated by qRT-PCR experiments.
This analysis was limited to ENC and OV MIX libraries as the number of genes to be considered in the SMART libraries was not statistically significant.
To circumvent this issue "small-intelligent" or "smart" libraries have been developed to provide equal frequency of each amino acid without bias.
The pool of domains used to search for TCS proteins was manually selected from the literature [ 4, 5, 8- 11] and extracted from within the Pfam and SMART libraries.
However, large clones are rarely found in SMART libraries as well as in libraries constructed according to the other full-length enriching techniques [ 8, 9], unless specialized lambda vectors are used [ 10].
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