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Fluorescent dye-labelled probes for hybridization were produced with SMART fluorescence probe amplification kit (BD Biosciences).
The relative sharp emission highlights the outstanding superiority of the luminescent perovskite NCs over the traditional smart fluorescence materials.
Positive colonies were detected by green fluorescence using a JuLI™ Smart Fluorescence Cell Analyzer (NanoEnTek Inc, Seoul, Korea), then collected and grown following the same procedure as above with the same media used for selection.
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After magnetic separation from test solution, positively charged Ag@SiO2 NPs were added, they were electrostatically absorbed on negatively charged DNA chains, acting as "smart dust" to enhance fluorescence signal.
Using an NIRF imaging "smart" probe, the presence and distribution of fluorescence were examined in arthritic joints of mice by noninvasive fluorescence [ 87].
Herein, a smart single-stranded DNA binding protein (SSB -assisted fluoreSSB -assistedr switch based on fluorescence resonaptamerergy transwitchFRET) was designed.
Quantification was performed densitometrically in μg per 1 × 10 cells using a Chemi-Smart 500 (PeqLab, Erlangen, Germany) for fluorescence detection and the CS4 Windows software for calculation of luminescence intensities.
These smart labels triggered a plethora of advanced fluorescence methods to scrutinize biological cells or organisms dynamically, quantitatively and with unprecedented resolution.
Especially, the novel Xn-GP composites with fluorescence property have the promising applications as smart electrode materials for supercapacitors.
To minimize the background signal and solve the problem that a variable quantity of DNA has different fluorescence intensities in every reaction tube, we devised a smart way to accurately standardize between samples.
In particular, a smart mathematical model, optimizing the integration of all the analysis steps of the fluorescence data from on chip multiple real time PCR, is described.
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