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The SMART assay using a synthetic model influenza DNA target sequence served as a fundamental demonstration of the efficacy of the capture and microfluidic separation system, thus bridging our system to a clinically relevant detection problem.
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(f) Invasion assay using Matrigel.
We performed this assay using the QCM™ Gelatin Invadopodia Assay according to the manufacturer's instructions.
Figure 1: MiRNA-mediated gene-silencing assay using reporter plasmids.
Similar results were obtained from assays using radiolabeled vesicles.
Creatinine was assayed using a spectrophotometric assay.
For the SMART-MSP assays the standard dilution series were, in addition, used to assess the quantitative accuracy and the PCR efficiency of the assays using the LightCycler 480 Software Version 1.5.
LRN PCR assays using the BA1, BA2, and BA3 primer and probe sets were performed with the LightCycler (Roche Diagnostics GmbH, Mannheim, Germany), Smart Cycler (Cepheid, Sunnyvale, CA), or ABI Prism 7700 (Applied Biosystems, Foster City, CA) instruments.
Assays used were TaqMan Gene Expression Assays (Life Technologies).
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