Our standard cutoff, half the size of the smallest normal cell, seems reasonable, but it should be recognised that it is not entirely satisfactory.
The threshold of 16 μm (two-fold the diameter of a filter pore and the size of the smallest normal cell) was set to exclude most of the normal circulating blood cells.
However, if the cutoff used was just below the size of the smallest normal cell (at a value of 290), which is also reasonable, then the calculated % apoptosis would be only 13.8%, which represents a reduction of 36%.
These results demonstrate that 'apoptotic cells' are smaller than normal cells, and that many are sufficiently small that they must be considered subcellular.
In most of the data shown, we used a threshold of approximately 1/2 the size of the smallest normal (untreated) cell, which was chosen to reduce the possibility that a single cell could generate multiple fragments that would be counted as cells.
La-A-treated cells, mostly round in shape and smaller than normal cells, were gradually separated from the duct-like structures (Fig. 4B).
He, however, described it as a 'respiratory impairment' due to the fact that, in cancer cells, mitochondrial respiration is smaller, relative to their glycolytic power, but not smaller relative to normal cells.
Wing size reduction is associated with both apoptosis and a smaller than normal cell size.
When mixed with a small proportion of normal cells ( <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0010909.e039.PNG" class= inline-graphic"/> ), the performance of GenoCNA is comparable with MixHMM except for its low recovery (0.46) of states with more than three copy numbers.
PtAcD differentially activated JNK and p38: such activation is prolonged and strong in tumor cells whereas transient and of the small intensity, in normal cells.
