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Of the four hexamerins assayed by RT-qPCR hex 70b showed the smallest fold change between queen and worker larvae (25-fold, Figure 3A).
To reduce the risk of false positive selection, we limited the smallest fold change (ratios AC/AT or AT/AC) of mean expression signals in the paired data to higher than 1.2 and the bootstrap method p-values to less than 0.01.
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Not in the top ten were genes D and F, which had the smallest fold changes (Fig. 2a).
Because we assigned significance to a relatively small fold change (1.5 fold) for quantification, we verified the regulation of two adipocytokines that changed by 1.5 fold by an independent, orthogonal method.
Some of these genes, and others with smaller fold change yet of particular interest, are described below.
We further excluded all probes with a small fold change (less extreme than +/− 1.5 fold) which results in 750 probe sets.
These genes have relatively small fold change (1.25 – 1.5), but the combined increase of multiple genes in this pathway strongly suggests that the ER stress response is not insignificant.
For instance, a small fold change in some lowly expressed gene may have greater biological importance than a small fold change in some abundant gene.
The text has been edited to accurately reflect the small fold change.
Many significantly down-regulated genes have small fold change, especially after 4 hour E2 treatment.
The expression of MTS1338 was repressed in our study, and showed a small fold change (logFC = −2.03).
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