Similar absolute values of phenolics to those reported by Meot-Duros and Magné (2009) for leaves sampled in spring were determined for genotype FR, while genotype PO, representing the smallest phenotype with low biomass production, exhibited a significant lower total polyphenol and ASC concentration (Figs 2, 4 and 5).
In all strains, Sirt1 deficient mice have a small, feeble phenotype, with significantly increased post-natal mortality rates.
(D ) Representative images showing the small nuclei phenotype, with single nucleoli following depletion of Ki-67.
The Verhulst death probabilities depend on the phenotype, and so does the mating preference: females prefer mating partners with the largest (smallest) phenotype, if their own phenotype is larger smaller) than average.
The most common, by far, phenotype scored in our screen was the MG (more and smaller Golgi spots) phenotype, with 41 out of 49 hits displaying it to different degrees (Figure 8C).
Homozygous mice with deletion of the floxed neor gene (R49C/R49C) have a more drastic phenotype, with smaller eyes and smaller lenses than wild type and heterozygous (WT/R49C) mutant mice [ 55].
In our positive gene set, C53A3.2 and skr-8 have been previously shown to have a synthetic small brood size phenotype with daf-18, an indication of poor germ line proliferation [34], [55].
In comparison, the small bouton phenotype associated with VAP wt) expression was reversed with Tsc1 knock down as well as S6K-CA expression.
As reported previously (Chen et al., 2003, 2005), MFN −/− MEFs exhibited a mitochondrial fragmentation phenotype with small globular mitochondria scattered throughout the cytoplasm (Fig. 8C), a smilar phenotype is seen in HeLa cell depleted for endogenous Mfn proteins (Eura et al., 2003, 2006), supporting the conserved function of mitofusin proteins in HeLa cells and MEFs.
Therefore, we developed lens specific RLIP76 transgenic mice (lensRLIP76 Tg) to elucidate the role of RLIP76 in protection against oxidative stress, but these transgenic mice showed impaired lens development and a phenotype with small eyes similar to that observed in microphthalmia.
