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In the smaller alignment, manual adjustment was made to the C-terminal region around the conserved "P[ILV]NK" motif [141], and the Cys residues of VirB7Ti and Rickettsia VirB7 were homologized (Figure 8A).
This resulted in a smaller alignment of the remaining 54 species (missing data 13.97%).
The smaller alignment was used in the Bayesian analysis that was very time consuming.
On the other hand, higher SNRs result in smaller alignment errors, which then lead to decreased blurring in the reconstruction.
In vivo small stretching (23%) employed in our study may cause a smaller alignment of reticular collagen bundles.
A second smaller alignment, representing 30 taxa, was constructed by removing sequences with large amounts of missing data, as well as sequences demonstrated to produce phylogenetic artifacts in previous analyses.
Similar(53)
To obtain such estimate, the alignment was repeatedly randomly sampled to create smaller alignments of varying size, whose entropy was measured.
First, we wanted to determine a threshold for subdividing sequences into smaller alignments.
We ran the algorithms on the full alignment and on two smaller alignments created by randomly sampling 20000 and 40000 sequences from the large alignment.
The global alignment was divided into 37 smaller alignments, which covered nearly the whole gene, whilst ensuring that none of the HMMs included exon borders; we then used HMMER to construct the protein HMMs from these alignments (Additional file 9).
Multiple localizations to a single exon can occur because masking or mismatches within exons can split what should be one long matched segment into two or more smaller alignments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com