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The developed biosensor showed good sensitivity, fast response and reproducibility with a small volume of sample.
The development of Luminex multi-analyte profiling (xMAP) technology helps overcome these obstacles by allowing for the simultaneous measurement of multiple analytes from a small volume of sample.
This device develops pressure in excess of 50 GPa by squeezing between two diamond gems a metal washer containing, in the central hole, a small volume of sample.
In our previous study, the possibility of using chloride ion sensitive fluorescent dye to determine the chloride ion concentration with a small volume of sample has been explored.
The biosensor assay exhibited a linear response to EDCs with a low detection limit of 2.4 2.9 nM (S/N=3), and required only a small volume of sample (1.5 µl) with the assay time of 2 h.
Compared with previously reported methods, the current method requires a small volume of sample and simple sample preparation steps for sensitive determination of NSAID levels using a conventional GC MS system.
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A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera.
Among immunosensors, electrochemical immunosensors are attractive and have received considerable attention because of their great features of being easily used and economical in mass production; having an excellent LOD with a small volume of samples; and being a paper-based simple analytical platform.
The developed method allowed the use of small volumes of sample and solvents and rapid separation by using a fused core column (only 2.2 min were needed).
Where only small volumes of sample solution exist, vial inserts can be used allowing samples to be analysed from as little as 20uL of solution.
This assay could be useful especially for the clinical research because small volumes of sample allow to several cytokines' information at the bedside.
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