Sentence examples for small volume of cold from inspiring English sources

The method is based on the preservation of intact microtubule arrays during cell lysis in a large volume of buffer, followed by disassembly of microtubules in a small volume of cold buffer.

Before tissue dissociation, fat and necrotic areas were dissected from surgical materials, and tissue samples were minced in a small volume of cold HBSS into 1 mm pieces using sterile scissors.

After centrifugation, the pellet was dissolved in a small volume of cold PBS (Euromedex, Souffelweyersheim, France), loaded on a discontinuous sucrose gradient constituted of 5 ml of 60%% sucrose in PBS (w/w) and 5 ml of 20%% sucrose in PBS (w/w), and centrifuged for 2 h at 200,000 g.

Reactions were performed in 10 m M Tris pH 7.0, 5% DMSO at 25°C, initiated by the addition of a small volume of cold, dilute enzyme (typically 10 n M), and the initial rates of formation of the 2- and 4-hydroxylamine products of CB1954 were monitored spectroscopically at 420 nm, a wavelength at which both products have the same molar absorbance (1200 M−1cm−11200 M−1cm−1

The pellet was resuspended in a small volume of ice-cold buffer containing 10 mM Tris-HCl (pH 7.6), 10% (v/v) glycerol, 1 mM DTT, and 1 mM EDTA.

Cells were then suspended in a small volume of ice-cold PBS, typically 200 – 400 µL depending on the estimated amount of cells isolated.

The final pellet was resuspended in a small volume of ice-cold distilled water and stored at 4°C until use.

Red cell lysis was performed by incubating the cellular pellet with a small volume of ice-cold distilled water for 20 s, followed by quenching with a large volume of isotonic PBS.

Why can pasta cook normally in a small volume of water that starts out cold?

The ELP pellets were dissolved in a tenfold smaller volume of ice-cold Milli-Q water and centrifuged at 4°C for 45 min and 15,000 rpm in an SS34 rotor.

However, evaluation of the combined effects of exposure to contaminated water and volume of cold water consumed was limited by the small number of subjects in this study.