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They had a mean diameter of 14.57 µm (in suspension) and appeared morphologically as small, spindle shaped, fibroblast-like cells (Fig. 1D, E).
For the fibroblast morphological analysis, three differentiation stages were evaluated: FI = small spindle shaped cell, FII = small epithelioid cells, FIII = large epithelioid cells (Bayreuther et al. 1988).
LM38-HP small spindle cells were positive for both pan-CK and vimentin (data not shown), but negative for α-SMA (Fig. 1, middle panels).
The cells from Lrp6 +/− and Lrp6 −/− mice lost their small spindle shape and turned into elongated bigger size when the same numbers of cells were seeded for each group and cultured for several days.
Histopathological examination of lesions showed epithelial covered, well-vascularized, cellular fibroblastic tissue comprised of small spindle to stellate fibroblasts with small dark basophilic nuclei dispersed in a dense collagen matrix.
In contrast, s.c. injection of the LM38-HP cell line, composed of a predominant small spindle epithelial subpopulation and a minor subpopulation of morphologically altered myoepithelial cells, resulted in the formation of undifferentiated solid carcinomas with a poor stroma.
Similar(46)
The largest "spindles" are about a foot long (30 cm), but most are smaller; small "spindles" have virtually the same shape as larger ones.
a HE staining shows tumor cells with small spindle-shaped cytoplasm.
Grade 0 represents tumors with less than 10 fibrocytes per high-power field characterized by small, spindle-shaped nuclei.
In 1% FBS containing StemPro-34SFM, small spindle-like cells were expanded, and formations of small colonies were observed.
The center of the nodules contains a preponderance of small, spindle-shaped cells, whereas epithelioid cells with large cytoplasm predominate in the periphery.
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