Interestingly, the structural variations followed a pattern similar to small sequence variations (Table 8).
Detection following a multiplex PCR is based on solid phase hybridization in microtiter plate format using general and type-specific probes at medium stringency, which makes the detection robust in case of small sequence variations.
The development of prediction methods is needed because even current high-throughput techniques, such as SPOT, are limited to rather small sequence variations [16].
The ligation step is able to discriminate even small sequence variations in the genome [ 1].
Small sequence variations in genes were detected by amplification of fragments containing individual exons and their splice intron exon junctions, and sequencing.
The present study delivers valuable information from more than 6,000 chickpea transcripts that present small sequence variations, and nevertheless very different expression levels under salt stress conditions.
We find that syntenically conserved tRNAs genes are typically conserved with an identical sequence across species, even though some tRNAs with the same anticodon located elsewhere in the genome show small sequence variations.
As shown in the alignment of pre-microRNA sequences in the largest previously defined microRNA family within the Dlk1/Gtl2 region [ 19], mir-159 (see Additional file 3), these differences are due to small sequence variations, shifts in the hairpin structures causing different cleavage sites and differences in the location of the mature microRNA within the hairpin (5' vs. 3' end).
Studies of H3.3, a histone variant that differs with canonical H3 by merely four amino acids and that plays a critical role in embryonic development and gene expression in adulthood, illustrate how small sequence variations in histones can generate distinct functions (Elsaesser et al., 2010).
