Sentence examples for small scale protocol from inspiring English sources

Expression was then carried out as for the small scale protocol.

A small-scale protocol from Affymetrix (High Wycombe, UK) was used to reproducibly amplify and label total RNA.

The effect of sugar mixtures (glucose, xylose, inducer) on the secretion of cellulolytic enzymes by a glucose-derepressed and cellulase-hyperproducing mutant strain of Trichoderma reesei (strain CL847) was studied using a small-scale protocol representative of the industrial conditions.

Plants were grown axenically on solid media and in liquid culture according to the small scale cultivation protocol as described [ 45].

For petals and sepals RNA was extracted using the Pure-Link Plant RNA Reagent small scale RNA isolation protocol (Ambion, Austin, TX).

In addition, the cost-of-goods is an important aspect which is often overlooked at the early stages of protocol development in small scale.

First we wanted to pilot focus group interview protocols on a small scale before undertaking large-scale interviews.

This picogram-scale protocol should be broadly useful not only for small scale ChIP experiments, but for any high throughput sequencing experiment where material or yields are limiting and multiplexed sample analysis is desired.

Briefly, the Saccharomyces cerevisiae reporter strain AH109 was transformed simultaneously with certain combinations of pGBKT7-based and pGADT7-based fusion constructs using a small scale lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/PEG) transformation protocol [58].

Small scale experiments (not using 1536-well platemployedoyed a previously described protocol for measuring GLuc activity, using either a Promega GloMax plate reader or a single tube Monolight 3010 luminometer.

In[25], a protocol which takes into account the channel quality (large and small scale fading, and thus SNR) on a hop-by-hop basis to select the best route from source to destination was presented.