Exact(1)
One approach to validate recovery or accuracy is based on small scale preparations of analyte in vehicle which mimic formulation preparations.
Similar(59)
For the EMSA experiments shown in Figure 4: (A) Control 'nuclear extracts' correspond to 1 μl of 0 6 hr nuclear extracts (corresponding to about 2.5 mg of embryos) from the small scale preparation described above.
It addresses resources, procedures, and documentation for small-scale preparations.
The large scale assays were also consistent with the synthetase activity observed in the small-scale preparations for these substitutions.
Small-scale preparations at nonindustrial sites (hospital pharmacies, nuclear medicine departments, PET centres) indeed represent an increasingly important segment of application.
Small-scale preparations of plasmid DNAs were done using the QIAprep Spin Miniprep Kit (QIAGEN Inc, Valencia, CA, USA) or the alkaline-iodine lysis procedure [ 26].
For conventional small-scale preparations, the reactions were carried out in Wheaton 1.0 mL v-vials (clear glass) assembled with TFE/silicone septa.
A scaled-down version of the protocol was used to extract DNA-PK from HeLa cells from a single 15 cm dish, showing that small-scale preparations of DNA-PK can be rapidly made (Fig. 2C).
For small-scale preparations, 50 μL of a haloarchaeal cell suspension, which was stained with the LIVE/DEAD® BacLight™ bacterial viability kit (Invitrogen, Lofer, Austria) as described previously (Leuko et al., 2004), was dried onto a glass slide for 2 3 days at ambient temperature in the dark.
The current methods, e.g., precipitation or ion exchange, are for small-scale preparations from relatively pure solutions (Liu et al. 2011; Buchert et al. 1986; Devos and Huchette 1981) and would not be adequate for bulk production of d-xylonic acid required for use as a platform chemical, e.g., for polymer or hydrogel production.
Arabidopsis DNA was prepared as described [14], using a small-scale preparation method.
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