Sentence examples for small scale expression of from inspiring English sources

Based on the analysis of cell lysate from the small scale expression of five antibodies, we have determined that best expression levels can be obtained using only two sets of conditions: for PA38, PA64 and 4G2, optimal expression was observed from the high copy plasmid, induced at OD600 1.0 with 20 ng/ml inducer.

This single-round panning allowed the elution of clones, of which two hundred and eighty eight were randomly hand-picked and small-scale expression of scFvs was induced.

Small-scale expression of all five candidates from the pCold-II-based vector suite gave significant amounts of recombinant protein, with the exception of the TRX fusion constructs (Table  3).

A random selection of resulting colonies was screened for small scale expression.

Emerged colonies were used in a small scale expression assay, where each colony was used to inoculate 10 mL of YPG/Zeocine 100 μg/mL liquid medium.

Alternative methods to quantitate small scale expression may include bead capture via the His tag, elution, followed by quantitation of the purified protein or ELISA based screens.

Titre reached up to 3.05 μg mL−1 in small scale expression.

After yeast transformation, a small scale expression assay was performed.

To analyse the protein expression levels of the selected clones, a small scale protein expression screen was performed.

Some overexpression systems like Pichia pastoris display often impressive levels of MP production at a small scale but expression at a larger scale is tricky and requires sophisticated devices [44].

For small-scale expression, volumes of 30 mL were used, while large-scale expression was performed in 500 mL LB medium.