Sentence examples for small scale expression in from inspiring English sources

Prior to use for requested target proteins, every new vector was validated with eGFP for cloning and small scale expression in the respective host.

In order to optimise the conditions for the protein expression preliminary small scale expression tests were performed in 24-well plate format.

Titre reached up to 3.05 μg mL−1 in small scale expression.

Western blots from the small scale expression show a clear difference in expression levels among the different clones.

Emerged colonies were used in a small scale expression assay, where each colony was used to inoculate 10 mL of YPG/Zeocine 100 μg/mL liquid medium.

After yeast transformation, a small scale expression assay was performed.

Functionality in cloning and expression was controlled for each new vector by eGFP SLIC cloning and small scale test expression in the appropriate host.

In this chapter, we provide protocols for both large-scale receptor expression in P. pastoris for structural studies and small-scale receptor expression in S. cerevisiae for functional characterization.

The small sample size of the study could have contributed to failure to detect such a difference and also other small scale changes in gene expression.

Colonies were screened for expression through small-scale expression experiments in 24 well deep-well plates containing 2 ml YPNM medium.

High-throughput protein expression is routinely performed by structural genomics projects to test expression clones, usually involving small scale cultures in microtiter plates [ 4].