Sentence examples for small scale cultures from inspiring English sources

Briefly, small scale cultures were induced in exponentially growing bacteria (A600 = 0.6 0.8) with 0.1 mM isopropyl -D-thiogalactoside for 20 h at 18°C.

Small scale cultures of 25 mL were grown overnight at 28°C in LB and selected antibiotics.

7A10B2 scTCR Vαβ and Vβα in pFKPEN was produced in several small scale cultures (10 × 50 ml) without any additives.

However, by pooling the periplasmic fraction from several small scale cultures, yields of μg/L were obtained.

Small scale cultures were grown in 250 ml Erlenmeyer flasks containing 50 ml medium or substrate and incubated at 30°C, 200 rpm.

High-throughput protein expression is routinely performed by structural genomics projects to test expression clones, usually involving small scale cultures in microtiter plates [ 4].

We successfully transduced Fabry patient CD34+ hematopoietic cells with "near-clinical grade" LV/AGA in small-scale cultures and then validated a clinically directed scale-up transduction process in a GMP-compliant cell processing facility.

Though some mutations clearly dampened protein expression, deletion of amino acids 57 70, corresponding to the predicted 3rd alpha helix of α-mating factor secretion signal, increased secretion of reporter proteins horseradish peroxidase and lipase at least 50% in small-scale cultures.

These small-scale cultures were compared for levels of expression of the GST fusion protein by anti-GST Western blot.

This will lead to balanced pH-regulation in small-scale cultures in the future.

Small-scale cultures were illuminated by 250 μmol photons s-1 m-2.