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Moreover, MnP was overproduced 3- to 4-fold in wheat bran (data not shown) and a 40% increase in compost was observed in transformants MnP1-1 and MnP1-2 relatove to A15 in a small scale cultivation (data not shown).
Before being confined to reservations, the Yavapai were mainly hunter-gatherers, following an annual round, migrating to different areas to follow the ripening of different edible plants, although some tribes supplemented this with small scale cultivation of the "three sisters" – maize, squash, and beans – in fertile streambeds.
Plants were grown axenically on solid media and in liquid culture according to the small scale cultivation protocol as described [ 45].
In order to investigate the distribution of carbon from the two carbon sources (D-glucose, L-glutamate) present in medium SSBM-P, we performed small scale cultivation experiments (200 mL) in custom made fully controlled and monitored mini-fermentors.
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Due to its fast growth on model compounds (ferulate, p-coumarate and benzoate) in M9 media during small scale cultivations (not shown), isolate 9.1 was selected for further characterization in the present study, and its growth on six lignin model compounds (ferulate, p-coumarate, benzoate, syringate, vanillin and guaiacol) was assessed.
Therefore, specific online-monitoring tools for small scale cultivations (RAMOS, BioLector) as well as a novel cultivation platform (Robo-Lector) were used for rapid process optimization.
Therefore, the influence of phosphate on growth and recombinant protein expression (at various induction times) in batch cultures was studied with specific online-monitoring tools for small scale cultivations, namely RAMOS [ 28, 29] and BioLector [ 30, 31].
However, with conventional techniques (e.g. small scale cultivations in shake flasks), it is very tedious to establish optimal conditions for cell growth and protein expression, as the start of protein expression (induction time) and the degree of phosphate limitation have to be determined in numerous concerted, manually conducted experiments.
Small-scale cultivation and traditional pastoralism are the main forms of agricultural activity.
The landless peasants see Mr. Lugo, who lived and worked as a priest here in San Pedro for 11 years, as their best chance in decades to help them win back land for small-scale cultivation.
However, the coalition has allowed small-scale cultivation trials to take place.
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