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So, in the spirit of being a good neighbor, a small primer, shout-out to and meditation on our friend the moose.
If you need to refresh your memory on where we last left off with Agent Dale Cooper and the wacky characters of Twin Peaks, we've compiled a small primer to get you caught up.
In addition to providing superior alignment (Table 1), by ignoring small primer errors Aptaligner finds 40 100% more counts per random region total compared to exact primer matches (Exact Match).
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Three small primers were used: T3GC (5' CCCAKTCGTGAWTCATGCT 3'), T3R, (5' TCCTCAYTTAATNAMCATGCT 3') and Rsh (5' ATCAAAAT 3').
We did not detect any smaller primer extension products, suggesting that the LNA cotransfection did not induce transcription of shorter mRNAs.
Exon 1 was too small for primer design in the exonic region; therefore primers flanking the exonic region were designed.
The cDNA was amplified over 15-18 Pcyclesles to produce sequencing libraries using Illumina's small RNA primer set.
The lack of a difference could be because the sample sizes were small for primer pairs with one or more IS-mismatch.
The cDNA was amplified by PCR with the Illumina small RNA primer set and sequenced on the Illumina HiSeq 2000 at BGI (Beijing Genomics Institute, Shenzhen, China).
Next, the cDNA was PCR-amplified for 20 cycles using Illumina's small RNA primer set and Phusion polymerase (New England Lab, USA).
The cDNA was PCR-amplified with Hotstart Phusion DNA Polymerase (New England Lab, USA) in 15 cycles using Illumina's small RNA primer set.
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CEO of Professional Science Editing for Scientists @ prosciediting.com