Years before I took my exam, a student in my graduate lab glued an Erlenmeyer flask to a small pedestal, creating a lab trophy.
Fill your beaker or Erlenmeyer flask with a small amount of distilled water.
Initially, a culture was kept in artificial seawater in an Erlenmeyer flask and fed with a small piece of raw beef.
Small scale cultures were grown in 250 ml Erlenmeyer flasks containing 50 ml medium or substrate and incubated at 30°C, 200 rpm.
Finally, actively growing bacterium-free hairy roots were cut into small segments and routinely cultured and refreshed in Erlenmeyer flasks (250 mL), containing 30 mL of sterile liquid B5 medium supplemented with 3% sucrose and 1% of PVP, sealed with a double layer of aluminium foil, and placed on an orbital shaker at 95 rpm in the dark at 25 ± 2°C.
Schöniger flask combustion involves a special Erlenmeyer flask to which is added a small volume of a suitable solution (often a dilute sodium carbonate or sodium hydroxide solution) that absorbs and retains the inorganic analytes.
Each length of the small diameter tubing ran to a separate 125 mL Erlenmeyer flask containing 100 mL of the appropriate reaction solution.
Each incubation sample weighing ~50 g of oven-dried soil was added to each Erlenmeyer flask that was covered by rubber stoppers with small holes to reduce evaporative water loss and maintain gas exchange.
A small volume of such seed culture was inoculated to each 150-mL Erlenmeyer flask containing 50 mL of the fermentation medium (FM) containing (g/L) yeast extract, 7; Peptone, 2; (NH4)2SO4, 2.05H2PO4, 2.0.25Na2HPO4, 0.25 to make an initial inoculum size of 0.5 OD60.5
Small agar plugs (approximately 5 × 5 mm) with the fungus were cultured in 0.5-L Erlenmeyer flasks containing 100-mL potato dextrose broth (PDB, potato infusion of 200 g fresh potato, dextrose 15 g, distilled water 1.0 L, pH 7.0) at 130 rpm and 28 °C for 3 days.
