One of the most remarkable findings of these studies is that relatively small degrees of sequence diversity, e.g., 10 different random sequence for esterase activity, are sufficient to allow Darwinian selection of random polypeptides composed of about 140 amino acid residues [17].
In this study, about 14% of the small molecules identified by virtual screening were shown to have a degree of antagonism, remarkable considering the receptor structure was not an experimental structure but a homology model built from a related structure with a low degree of sequence homology.
The low degree of sequence polymorphism within TvMULE families suggests a very recent expansion of Mutator-like transposons in the T. vaginalis genome, either due to TE-induced proliferation or to small-scale duplications of the host genome.
The small degree of overlap between the screens further suggests that sequence differences between the ade2 -min3 and ade2 -h7.5 minisatellites may govern repeat tract stability and alteration.
Previous studies performed by comparison of different DNA sequences such as housekeeping genes and multiple tandem repeats, and more recently whole genome sequencing, have shown a small degree of genetic diversity between isolates obtained throughout the world [ 8- 11].
The tree inferred for the minor segment (Figure 4A) supports a small degree of phylogenetic incongruence, but not indicative of homologous recombination of the given parental sequences, or any other sequences in this tree.
Due to the very small degree of freedom resulting from restrictions on oligonucleotide locations, the design targeted the best possible oligonucleotide for each sequence rather than imposing a strict oligonucleotide quality cutoff.
This is causing the White House no small degree of concern.
