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Moreover, in IP assays, SpA-SFP@SiO2 showed an approximately 300% higher precipitation of target protein than the commercial SpA-Agarose product when a small amount of cell lysate was used.
Real demonstration of nonlytic spread for poliovirus or any other cytoplasmic constituent thought to exit cells via unconventional secretion requires demonstration that a small amount of cell lysis in the cellular population is not responsible for the release of cytosolic material.
Total cellular RNA was isolated using Trizol reagent (Invitrogen), or RNeasy Micro Kit (Qiagen) for a small amount of cell samples according to the manufacturer's recommendations.
In the PBS combined with PTT + RT group, a small amount of cell shrinkage and karyorrhexis was observed ((Fig. 8c) I-8(C) III).
A small amount of cell lysis was observed in Hepa-1c1c7 cells treated with HI-NHS and either the AdCAGGFP-conditioned media (10.68±1.27%) or the AdCAGsCD59-conditioned media (11.03±1.92%), which could be the result of cell manipulation during the experiment (Figure 2B).
This technique allows the use of a small amount of cell culture to evaluate its genotype.
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The small amount of cell-free fetal DNA (cffDNA) can be a useful biomarker for early non-invasive prenatal diagnosis (NIPD) of achondroplasia.
However, their use is limited by expansion capacity and the small amount of cells that are obtained.
However, for tracking small amount of cells, MR sensitivity of the commercially available SPION agents are still relatively low.
Since the tumor cells are heterogeneous [18, 19], the mutations occurred on a small amount of cells could be covered by the other normal cells [20].
Biological microelectromechanical systems (Bio-MEMS) present a suitable approach for analyzing a small amount of cells on a defined cell culture area.
More suggestions(15)
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