In summary, our study demonstrates that expression profiles can be obtained in a reproducible manner from the small amount of RNA obtained from breast tumor FNAs.
However, this was not confirmed by sequencing because of the small amount of products obtained after the amplification reaction.
In-situ SBM was designed to analyze methylation status of genes in small amount of DNA obtained from formalin fixed PEAT.
One limitation of this study was the small amount of CSF obtained from a single rat, which prevented the analysis of individual animals to determine the degree of inter-individual variability in the proteins levels following injury.
The large number of non-specific bands, combined with the small amount of protein obtained from siRNA experiments and difficulties in solubilizing PINK1 [29, [29] meant that reliable detection of PINK1 protein expression following siRNA treatment was not possible.
Although it should be noted that this was an exploratory study and the conclusions should be interpreted cautiously due to the heterogeneous cell population from the nasal biopsy and the small amount of material obtained, which did not allow for any validation of gene expression differences.
A major challenge to the development of biomarkers for pancreatic cancer (PC) is the small amount of tissue obtained at the time of diagnosis.
As females in our study received only a single gift, it is possible that the small amount of nutrients obtained through one gift is insufficient to detect an effect of nuptial gift quality on female fitness traits.
In fact, FRET analysis allowed us to investigate the interaction of GM3 with TRAIL receptors also in B lymphocytes freshly isolated from pathological subjects, in which biochemical analyses were banished because of the small amount of cells obtained by drawing of a blood sample.
The most marked difference in the splicing pattern was detected for splice variant III; its expression in normal samples could not be detected by standard RT-PCR, probably due to combined effects of its very low expression level and a relatively small amount of the obtained cDNA, but in more than a half of the samples a clear PCR product was detected in tumor samples (Fig 4).
With 3-Cl-pyridine, only a very small amount of product is obtained with lower (92%) ee.
