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For SMA 1, it was 6.00 %, whereas for SMA 2 it was slightly higher (6.12 %).
SMA 1 mixture performed better than SMA 2 in this test.
For both SMA 1 and SMA 2 mixtures, very negligible stripping was observed.
SMA 1 mixtures have comparatively higher density, stability and other volumetric and Marshall properties.
The increased density of SMA 1 caused less air voids and this provided less OBC value.
Marshall stability was observed between 14.6 20.1 and 14.5 19.4 kN, respectively, for SMA 1 and SMA 2 mixtures.
Similar(43)
Together, these results suggest that loss of SMN function in Drosophila causes aberrant neuromuscular synaptic structure, mimicking the pathology of SMA [1], [54].
Primary antibodies were diluted in blocking solution as follows: RGS5 1 200, CD31 1:50, PCNA and SMA 1 500, myocardin 1 100.
Dako Ark kit (Dako, Carpinteria, CA, USA) was used for immunohistochemistry with mouse anti-smooth muscle actin (SMA; 1 1,000; Sigma) antibody.
Antibody targeting α-SMA (1 : 1000) was from Abcam (Burlingame, CA, USA).
The sections were incubated with primary antibodies to α-SMA (1 200, SPM332, Abcam), NS5A (1:20, 9e10) and Core (1 50, C7-50, Abcam0, Abcam) overnight.
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