The samples were frozen with liquid nitrogen slush, then transferred to a sample preparation chamber at −160°C and etched for 15 min at −85°C.
Fresh leaves of Begonia guixiensis (Peng et al. 20310) and B. longa (Peng et al. 20076) were dissected and attached to a stub, frozen with liquid nitrogen slush, and then transferred to a sample preparation chamber at −160°C and etched for 15 min at −85°C.
Intact Bd leaf sections were mounted on an aluminum stub by using O.C.T. compound (BDH), plunged into liquid nitrogen slush, and then transferred onto the cryostage of an ALTO 2500 cryo-transfer system (Gatan) attached to a Zeiss Supra 55 VP FEG scanning electron microscope.
The uterus was kept on ice slush until it was transferred to surgical field.
If using nitrogen slush, remove the coolant from the vacuum.
They are also accused of running a secret slush fund of options allocated to fictitious employees, which could later be transferred to real ones.
From the interaction zone several parts were selected and excised with a surgical blade as small agar blocks, and transferred to a copper cup for snap-freezing in nitrogen slush.
Samples were then concentrated to ∼70 mg/mL in a Centricon-50 concentrator (Amicon) as described previously, transferred to MCD sample cuvettes, and frozen in a liquid nitrogen/pentane slush.
But more serious allegations of bribery were dropped even though he had been fingered by Samsung's former chief lawyer, who spoke of a huge slush fund.Mr Lee has also been charged with transferring control to his 40-year-old son and heir, Jay Y . Lee by arranging for Samsung affiliates to sell shares to the younger Mr Lee at artificially low prices.
A special prosecutor in January launched a probe into corruption allegations after a former Samsung top legal executive said the conglomerate had created a slush fund to bribe prosecutors, officials and other influential figures to suppress criticism of a series of questionable stock transactions, conducted with the intention of transferring the chairman's ownership stake to his son.
