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Exact(48)
200 μL of the contaminant slurry was added to the plates as shown in Figure 1.
The initial total bacteria count was about 1.55 × 1010 CFU mL-1 wet substrate when the chicken manure slurry was added and mixed together with the shredded pineapple leaves, chicken feed and molasses.
Anti-FLAG agarose slurry was added to the supernatant and placed in a disposable BioRad column.
The protein A agarose slurry was added to the antibody bound chromatin solution and incubated at 4°C for 1 hr with constant rotation.
For precipitation of proteins coupled with primary antibody, the 50 ul of protein A agarose beads (50% of bead slurry) was added and incubated with gentle rocking for three hours at 4°C.
To a stirred solution of 19 (0.46 g, 0.94 mmol) in MeOH (15 mL) Raney-Ni (1.0 mL as MeOH slurry) was added, and the stirring was continued under a balloon of hydrogen for 17 hours.
Similar(12)
Then La(NO3 3·6H2O solution and the HNT slurry were added to AlCl3 supersaturated solution to form a suspension.
25 µL of a 1∶1 protein A-agarose slurry were added, and the samples were rolled at 4°C for another hour.
Following antibody incubation, 50 μl of protein A/G-Sepharose beads (50%% slurry) were added and rotated at 4 °C for 3 h.
Protein A/G plus beads (40 μL, 50%% slurry) were added to capture the immunoprecipitated chromatin complexes for 2 h at 4 °C.
To deplete plasma samples of BV and BV-bound VEGF, 100 μl of protein G slurry were added to 200 μl of plasma samples and incubated at 4°C for 4 h.
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