After the culture medium was removed, 100 μl DMSO was added and the sample was slowly agitated for 10 min for crystals dissolution at room temperature.
First, the polymer powders were gradually sprinkled into the solvent and slowly agitated at 30 rpm, in order to prevent particles from clumping on the surface.
Subsequently, membranes were blocked with 5% (w/v) milk powder in 0.05% Tween-20 buffer and slowly agitated for 1 hr at room temperature.
The extract was slowly agitated for 60 min at 4°C and centrifuged at 10,000 × g for 20 min, and the supernatant was transferred to new Eppendorf tube.
The plates with inserts were slowly agitated using a horizontal shaker (100 rpm; Biosan, Latvia) in the CO2 incubator for 1 h.
The cell pellets obtained after slow centrifugation were re-suspended in a solution of PBS and 10 μg/ml of TPCK-trypsin and slowly agitated at 37°C for 30 minutes to produce cell pellet wash.
Microcosms were kept in an environmental chamber (20 °C, 10 14 (L/D) h photoperiod), covered loosely with plastic to minimize evaporation, and slowly agitated on an orbital shaker (100 rpm) to maintain aeration and suspension of algae.
In the Agrobacterium-mediated transformation, the pretreated calli were transferred into flasks containing Agrobacterium suspension and agitated slowly for 2 hours.
Run the tap very slowly over the bowl and continue agitating every so often, both to remove sand from their shells and to force them to spit out any from the insides.
I fear that when the leaders of the nation shift the foundation of schools and society for the mere purpose of agitating each other, they are slowly disintegrating the nation.
Water was slowly applied to each mixture container, while gently agitating, until excess water was observed.
