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These experiments showed very similar behavior as rat hippocampal neurons with the exception that the slowing phase was much less pronounced and was only evident at much higher stimulus levels (see below).
Consistent with the need to use greater stimulation to see the slowing phase, mouse cortical neurons appear to have their acceleration phase shifted slightly to higher stimulus numbers compared to rat hippocampal neurons as well.
The Ca2+ dependent slowing phase we observed was more prominent at lower temperatures and agrees well with previous findings that show Ca2+ inhibition of endocytosis (Balaji et al., 2008; von Gersdorff and Matthews, 1994; Sun et al., 2002).
DOI: http://dx.doi.org/10.7554/eLife.00845.009 Using high-sensitivity pHluorin assays of synaptic vesicle endocytosis for individual neurons we revealed the presence of two phases of stimulus and Ca2+ dependence of synaptic vesicle endocytosis: an acceleration phase prominent for small stimuli and a slowing phase prominent for larger stimuli.
However these experiments revealed a clear and robust acceleration phase for endocytosis at physiological temperature with stimuli ranging from 5 AP to 25 AP and milder slowing phase for stimuli above this than that seen at 30°C, with a minimum endocytosis time constant in the vicinity of 25 AP at 10 Hz.
The prominent slowing phase of endocytosis present at 30°C however was still observed at 37°C but it required larger stimuli (300 AP) to show obvious (55 ± 20% [N = 7]) slowing when compared to 100 AP 10 Hz.
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Under normal conditions optokinetic stimulation always leads to slow phase eye movements following the direction of the stimulus.
But the primary calendar is now entering a slower phase.
But he rejected the notion that the US is simply entering a slower phase in its development.
During the slow phase, the grass becomes dull-colored or, if the weather is dry, yellow or brown.
Specifically, Tsipras asked for a 30% discount on VAT for Greek islands, as well as a slower phase out of the "solidarity grant" for poorer pensioners.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com