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This result showed the possibility of adaptation process in circum-maxillary structure during the slow phase in MME that does not occur in a protocol with only rapid approach (Işeri and Ozsoy [[16]]).
A minor slow phase in the repartitioning cannot be excluded, however.
It is also possible, however, that there is a minor very slow phase in the repartitioning process.
Taking into account the lower temperature in their study (5°C), these values are in good agreement with those of the slow phase in our experiment (10°C), which increased from ∼20 s−1 to 330 s−1 with [Ca2+].
Further evidence that the slow phase in this study represents a global change of the Tn complex is given by the considerations about switch-off kinetics induced by Ca2+-independent relaxation from rigor (see below).
The minimal model that accounts for the observation of a fast and a slow phase in the "on" experiments is shown by the three-state model (Scheme 1, upper), where kB is the second-order rate constant of Ca2+ binding, kD the rate constant of Ca2+ dissociation, and kon and koff the rate constants of regulatory switch-on and switch-off, respectively.
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