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All three type of cells were finally centrifuged at 400 g for 5 min and the pellet was collected, re-suspended in freezing medium (90% FCS, 10% DMSO) and was slow frozen at -80°C in an isopropanol bath.
In an early, large multicentre study with 14 000 cleavage stage slow frozen and thawed embryos it was determined that 73% of the embryos had at least half of their initial blastomeres still intact and the results showed clinical pregnancy and implantation rates of 16 and 8.4%, respectively, after transfer.
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Fifth experiment: vitrification with sucrose supplementation was compared to slow freezing.
"Slow" freezing method promoted greater VLPs preservation of 36 40% depending on the thawing rate.
The brain was rapidly removed and placed in OCT on dry ice to slow freeze the tissue to avoid cracking.
The material subjected to "slow" freezing was put into a freeze-dryer chamber at ambient temperature and frozen at 2°C/min rate until −35°C.
Vitrification was found to be more successful than slow-freezing.
The cost saving in countries using vitrification may be greater than in countries still primarily using slow-freezing techniques.
Vitrification is superior to slow freezing, which in turn is superior to ultra-rapid freezing.
In a first experiment, a standard vitrification protocol, previously designed for sheep embryos was compared to slow freezing of goat embryos.
Vitrification procedure was recently introduced in our laboratory as an alternative to slow freezing for oocyte cryopreservation.
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