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In many cases (particularly slow freezing events at high subzero temperatures), numerous isolated freezing events were observed (Figure 5).
Vitrification is superior to slow freezing, which in turn is superior to ultra-rapid freezing.
To identify factors that might affect the clinical outcome of oocyte slow freezing.
Slow freezing was employed vertically as well as horizontally through solutions and suspensions.
To compare mature human oocytes cryopreservation with slow freezing (SF) and vitrification (VT) in infertile couples.
Slow freezing is a simpler procedure with lower CPAs concentrations than vitrification28.
Two approaches are widely used in cryopreservation: equilibrium (slow freezing) and non-equilibrium (vitrification) cooling procedures.
After slow freezing to −80 °C, the vials were stored in a −80 °C freezer for two weeks.
Vitrification has theoretical advantages over slow freezing as no ice is created in the process.
To evaluate and compare vitrification and choline-supplemented sodium-depleted slow freezing of mouse oocytes.
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