Exact(22)
This was followed by slow agitation at 30 rpm for 40 min.
Optimal flocculation conditions (Tanfloc dosage 10.42 mg L−1, rapid agitation for 0.36 min at 568.88 rpm and slow agitation for 14.14 min at 12.1 rpm) were obtained using the response surface methodology.
Common truck mixer rotation procedure, and fiber addition routine, in Norway: "the drum is rotated at slow agitation speed, 2 6 revolutions per minute, while underway to the construction site, with no fibers in the batch.
During slow agitation speed, SAC-MBH did not spread in the solution properly, instead it conglomerated in the solution; thus, beneath the apex layers of adsorbent it hide many active sites so apex layer is only available for adsorption and in this process hidden layers are not present to participate in the adsorption process, since they had no proper contact with As V).
Anti-Tbx1 (Zymed Laboratories) or anti-Flag M2 antibody (SIGMA) was coupled to an amine-reactive gel (ProFound co-immunoprecipitation kit, Pierce) using slow agitation at 4°C O.N.
The lysate was clarified by centrifugation at 15 000 rpm for 10 min. 20 µl of HA-coated agarose beads (Sigma) were added before incubation under slow agitation at 4°C for 4 hours.
Similar(38)
The plates were then placed onto ice, and the skin samples incubated with cold 4% PFA for 20 min. After washing three times with cold PBS, the skin samples were incubated in cRPMI with 6 μg/ml goat anti-Lyve-1 antibody overnight at 4°C with slow, gentle, agitation.
Fermentation experiment was carried out in micro-aerobic and aerobic conditions in a 250 mL flask at pH 5.0 ± 0.5 and 30 °C under slow and constant agitation (100 rpm).
Each patient presented with headache, altered conscious state and papilledema associated with different neurological symptoms such as dysarthria, hemiplegia, pernicious vomiting, ideomotor slowing and psychomotorr agitation.
This gentle agitation, or slow mixing, is called flocculation; it is accomplished in a tank that provides at least a half hour of detention time.
Generally, symptoms of poisoning appear within three hours of ingestion of the mushroom as visual hallucinations, nausea, vomiting, stomach pain, diarrhea, irregular and slow heart beat and agitation.
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