Bioavailability of lysine of the blood meals was assessed in comparison to that of l-lysine HCL using a slope ratio assay experimental design.
Bioavailability of lysine determined by the slope ratio assay, in coarse and fine SM was 0.66 and 0.62, respectively, when using N retention as the response criterion and assuming a bioavailability of lysine from casein of 100%.
The efficiency (E) of PCR was calculated from the regression slope of the assay (E = 10-1/slope).
Binding capacities established by these two methods were also significantly correlated (r = 0.79; P < 0.001), with systematically higher values for the DCC assay (slope of the regression line= 1.47; Fig 1).
The titrations of the labelled protein with SUVs were performed with a new microplate assay (slopes, to be published elsewhere; for more details contact author TMJ).
Moreover, the substrate turnover rate (TR) for HRP NAV and ALP NAV can be estimated from the assay slopes and ε of resorufin (54 000 M–1·cm–1) and fluorescein (50 400 M–1·cm–1) via TR = slope/([enzyme]ε).
However, as observed before with other PHAIA assays, the slope was rather steep, limiting the dynamic range of the assay.
In the aPTT assay, the slope of the initial phase of the light transmission profile quantifies an abnormal BPW.
Assay performance was assessed by intra-assay variation (% CV), fold difference reliably detectable with P = 0.05 (based on log10 SD), assay linearity (slope and r values derived from the linear regression for the low, medium, and high spiked samples), and detection accuracy (recovery of nominal gravimetric concentrations).
Thus the lysis rate (traditionally called the rise), which we define as the increase in the number of released phages over time and is indicated by the slope of the burst assay curve, is greater for the ancestor at 37°C (time*phage interaction: F = 2.58, df =12,41, p = 0.012) and greater for the mutants at 42°C (time*phage interaction: F = 3.69, df =14, 49, p = 0.0003).
