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After blocking the endogenous peroxidase with 3% hydrogen peroxide (H2O2) in PBS for 20 min the cover slips were processed according to staining procedure of the manufacturer's protocol for Histostain plus kits, Zymed Laboratories (Invitrogen, CA, USA).
Neurons grown on cover slips (Bellco) were processed in an identical manner except that it was mounted on slides with 0.5 µg ml−1 DAPI diluted in fluorescent mounting medium (Dako).
Control cover slips were processed as described above except that no primary antibody was added to the solution.
For indirect immunofluorescence assays, T. gondii-infected HFF cells grown on 22 mm glass cover slips were processed exactly as previously described [63]).
The paraformaldehyde fixed cover slips were processed for staining as described [16] CXCR2 and LASP-1 were probed with rabbit anti-CXCR2 [29] and mouse monoclonal anti-LASP1 antibodies (clone 8C6).
Using a sterilized plastic cafeteria fork, a series of scratches were made on confluent cover slips and these were processed for indirect immunocytochemistry as described below.
Experimental strains recorded on FRP/SRP strips were processed to evaluate shear slip laws of tested specimens; energy fracture and failure load values are compared with theoretical values by simplified models for shear stress slip.
After imaging of the endogenous signals, the cover-slip was removed and the slide was processed for additional stains.
The remaining groups were processed at days 4 and 7 post-infection; for these groups, both cover slips and supernatants were processed in order to include all viable bacteria within the closed system [30].
But the city lost the vision thing about 50 years ago, as it slipped into what appeared to be an inexorable process of decline.The best evidence of how much things have improved in New York is the fact that its mayor is again thinking visionary thoughts about the future.
Prismatic slip and pyramidal I slip are found to be very active in this alloy.
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CEO of Professional Science Editing for Scientists @ prosciediting.com