Examples of endogenous mutagenic processes are DNA replication (i.e. polymerase fidelity and replication slippage), post-replicative DNA mismatch repair and methylation-mediated deamination of cytosines in CpG dinucleotides [ 5- 9].
Following this protocol, approximately two thirds of the induced post-slippage ERRAS cells survived for at least 9 days.
One post is dedicated to the "slippage" between candy and medicine that she has found in a close reading of the history of cough drops — hard candy in a socially acceptable form.
Prior to stroke, all mice displayed equal slippage between both forelimbs; however, at 4 days post ET-1 injection, mice displayed a significant functional deficit (P<0.05), with increased contralateral slippage relative to uninjured controls.
The proximity of the post-capital pin to the sciatic notch and the accidental slippage of one pin into this site place the sciatic nerve at risk.
Rainey's skin wounds, described as "skin slippage" by Dr. Lew, were the result of normal post-mortem decomposition, "exposure to a warm, moist environment" and friction or pressure placed on his body by medics or prison officials who were trying to revive him or when they moved his body.
A permanent frameshift mutation in microsatellites is caused by slippage of DNA polymerase followed by a lack of post-replication MMR (5, 6).
Cells eventually adapt to this nocodazole challenge and escape to a state, termed 'G1-like post-M phase', with interphase morphology with micronuclei ('mitotic slippage' phenotype) (Schimke et al, 1991, 1994).
Thus the polyploidy cells observed may represent a response to spontaneous DNA damage, replication fork collapse due to stalling in repetitive regions of the genome, defects in post-replication events such as decatenation leading to SAC activation and mitotic slippage, or a failure in cytokinesis following normal mitosis.
Here we propose a simple post hoc correction of the measured tearing energy, and an improved clamp design to prevent slippage.
