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Those aptazymes that were predicted to best fit the slip structure model were synthesized and assayed, and the best-designed aptazyme was activated 60-fold by FMN.
The slip structure model proved to be generalizable, and could be applied with equal facility to computationally generate aptazymes that proved to be experimentally activated by other ligands (theophylline) or that contained other catalytic cores (hairpin ribozyme).
Moreover, the slip structure model could be applied to the prediction of a ligand-dependent aptamer beacon biosensor in which the addition of the protein vascular endothelial growth factor (VegF) led to a 10-fold increase in fluorescent signal.
Even though the C6T sequence predominantly exists as a single major conformer, a minor fraction can exist as a slip structure.
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While scanning electron microscopy techniques were used for the observation of surface slip structures, site-specific focused-ion-beam thin foil preparation and scanning transmission electron microscopy techniques were employed for the imaging of sub-surface dislocation structures.
The three-slip structure will be faced in glass and corrugated stainless steel.
The C 1 and C 2 anomalies are the fault zone conductors of the main Ganos Fault and subsidiary oblique strike-slip structure in the south, respectively.
An ocean continent transform boundary developed off western Tasmania follows the trace of an older Paleozoic strike-slip structure (Avoca Sorell fault system) optimally oriented for reactivation during the final separation of Australia from Antarctica.
We show that the pro- and retro-shear zones that develop in a doubly-vergent two-dimensional wedge curve around the end of the velocity line-discontinuity to merge into the strike-slip structure that naturally develop, i.e. as a consequence of the imposed boundary conditions, along the edge of the wedge.
This domain is marked by the development of high angle, strike-slip structures [59].
Gravity spreading resulted in squeezed diapirs (and associated allochthonous salt) connected by variably oriented contractional, extensional, and strike-slip structures.
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