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GO term enrichment analysis was done using Yeast GO slim (process) at the SGD homepage (http://www.yeastgenome.org).org
Of those unigenes with GO Slim category assignments, the shared interface-specific Orthogroups are overrepresented for the GO Slim Function category "transcription factor" and underrepresented for the Go Slim Process category "transport".
Interestingly the Orthogroups shared in the interaction of T. versicolor with both hosts are overrepresented by "transcription factor activity" GO Slim Function terms (Additional file 4: Figure S3A: column B and 3D: column F) and underrepresented by "transport" GO Slim Process terms (Additional file 4: Figure 3: column B and 3F: column F).
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Yeast GO-Slim: Process was selected and all of the GO-Slim Terms were used.
A more recent study showed that there is a difference from a random null model in the fraction of gene pairs in the same GO-slim process only for neighboring pairs with a high level of coexpression (r > 0.4) [ 12].
The final annotation file was generated after gene-ID mapping, GO term assignment, annotation augmentation, and generic GO-slim processes.
As noticed during the slim classification process as well, peripheral proteins participate in lipid binding and especially phospholipid binding and, regarding biological processes, those showing an overrepresentation are the regulation of signaling, cell communication, and phosphate metabolic processes.
Among the represented GO slim biological process in the most 40 up-regulated UniTags in salt stressed nodules, transcription and regulation of biological processes were represented by the UniProt entries ELOF1_ARATH (Transcript elongation factor 1), C7AFG1_CICAR (NAC family transcription factor 4), Q2PJR9_SOYBN (WRKY27 transcription factor), and PHYA_PEA (Phytochrome A).
GO slim (biological process) statistics for the corresponding UniProt accessions are depicted in Figure 3.
We used GO (Gene Ontology) Slim (biological process with 37 categories; see Methods) to address the issue.
The top 40 most up-regulated transcripts in chickpea nodules after 2 hours of salt stress are listed in Table 2. GO slim (biological process) statistics for the corresponding UniProt accessions are depicted in Figure 3.
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