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The CateGOrizer tool was used for final reduction of GO terms complexity along with the Plant GO Slim dataset available from the CateGOrizer web [ 31].
The yeast GO slim dataset was downloaded from the ftp site of Saccharomyces Genome Database ftp://genome-ftp.stanford.edu/pub/yeast/literature_curation/.edu/pub/yeast/literature_curation/
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A dataset of SLiM-binding domains and SLiM-mediated interactions was manually curated from the literature.
Instead a false positive dataset of SLiM-mediated interfaces unlikely to be true was constructed.
Within the BioGrid interaction dataset, 11 153 SLiM-mediated interactions were identified compared to 1112 in the randomized network suggesting a FPR of 9.97%.
This information is employed by PAM-SLIM to compose the sample dataset to cluster the data.
The first consists of experimentally validated SLiM-mediated interaction data (SLiMDoM dataset) and the second is based on the Domino dataset, which is curated from the Domino database's experimentally annotated peptide domain interactions (for full results see Supplementary Table S5).
SLiMProb needs one or more SLiMs to search within the protein dataset.
Based on BLASTX matches, average protein percent identity was estimated for each GO slim category, either in the 1 1 1 ortholog dataset, or as in all best hits (homologs) against the An.
The use of a bulk load operation based on a static dataset to build an optimized Slim-tree is proposed in [17].
Table 3 and Dataset S4 provide GO (Drosophila melanogaster slim) protein annotations under Biological Process, Molecular Function and Cellular Compartment categories for the most significantly enriched annotation observed in each population.
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