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In normal squamous epithelium, there was slightly positive staining for miR-21, whereas ESCC showed strongly positive staining and 3(b)).
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A portion of mesangial cells was also found to be slightly positive for staining by the antibody (data not shown).
Immunohistochemistry of patient-derived GCTB tissue sections revealed that the cells are almost negative for c-Met, although some giant cells and some stromal cells exhibited a slightly enhanced red positive staining.
A slightly positive PTH immunohistochemical stain was noted, whereas the RT-PCR results were positive.
The immunoreactivity for all proteins was defined as follows: strongly positive, more than 50% of tumor cells stained; positive, more than 25% of the cells stained; slightly positive, more than 10% of the tumor cells stained; and negative, less than 10% of the tumor cells stained or tumor cells lacking immunoreactivity.
Long term-cultured cells showed weaker signals for CD49f, VASA, DAZl, and STELLA and stained slightly positive for VIMENTIN.
Figure 2 illustrates that on day 1 after transduction only the outer rim of the spheroid stained slightly positive for C28-sCE2.
Tumors with final staining scores of 0, 1, 2 4 and 5 6 were considered to be negative, slightly positive, moderately positive and strongly positive, respectively, as described previously [ 21, 22].
Positive staining was observed for all groups, with Pg LPS and Ec LPS groups being slightly higher.
In lung tissue an intense staining was observed in bronchial epithelium for TRPC3 and C6 while TRPC5 detection was only slightly positive in these cells.
But conservatives are slightly positive about it.
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