Note, that there is a slight sample drift, which causes cpVenus173 emission increases in (A ).
Examination of the imaging data showed, that the increase of acceptor emission in the guard cell analyses in Figure 3A-C was caused by a slight sample drift.
Notably, positive elastase staining and cellular infiltration were observed within the bulk cAAPEG hydrogel, made more evident with slight sample separation that occurred as an artifact of processing.
We have added text to the figure caption noting that a slight sample drift contributed to fluorescence signals in Figure 3A.
A comparison of the sex and age characteristics of the study participants and census data from the target population indicates that the former are broadly representative of the latter, except for a slight over sampling of females in urban areas [ 26].
The final score was calculated according to (3) and classified as follows: 0.0 2.9 (sample is nonirritant), 3.0 8.9 (sample is slight irritant), 9.0 15 (sample is moderate irritant), and >15 (sample is severely irritant).
However, band shifting is more slight for sample containing the saline solution.
We analysed the swabs for the presence of Bd with Bd-specific primers in rt-PCR following the protocol by Boyle et al. [33] with slight modifications: samples were run in duplicates and when the two PCR-wells retuned inconsistent results, the analysis was repeated.
After washing (twice, 20 minutes each, rt with slight stirring), samples were labelled with FITC-conjugated anti-mouse antibody (Sigma-Aldrich) at a 1 500 dilution for 1 h at rt and washed with water.
