Long-range intrachain contacts between stretches between residues 100 and 180 as well as a slight sampling of the more central regions of OPN by the two termini are depicted.
However, band shifting is more slight for sample containing the saline solution.
The quantitative fractions of aromatic molecules and functional groups constituting oil hydrocarbons in several samples were determined and their slight variation from sample to sample was shown.
Some organisms might have been lost during the approximately 1 meter long transfer to the sampling box on Alvin, probably resulting in slight under-sampling of rare species and slight error in species abundances.
We analysed the swabs for the presence of Bd with Bd-specific primers in rt-PCR following the protocol by Boyle et al. [33] with slight modifications: samples were run in duplicates and when the two PCR-wells retuned inconsistent results, the analysis was repeated.
After washing (twice, 20 minutes each, rt with slight stirring), samples were labelled with FITC-conjugated anti-mouse antibody (Sigma-Aldrich) at a 1 500 dilution for 1 h at rt and washed with water.
A comparison of the sex and age characteristics of the study participants and census data from the target population indicates that the former are broadly representative of the latter, except for a slight over sampling of females in urban areas [ 26].
