Exact(1)
Nucleic acid extractions were conducted with the Promega SV Total RNA Isolation System [59], with slight protocol modifications (exclusion of part IV. E. steps 4, 5) to permit extraction of both genomic DNA and RNA.
Similar(59)
Slight differences in protocol may contribute to the different coefficients.
In revisiting these experiments, we found that results are sensitive to slight variations in protocol that we have not been able to control.
The DPPH (1, 1-diphenyl-2-picryl-hydrazyl) bioassay was performed according to the slight modification of protocol of Said et al. [ 12].
This was due to a slight change in protocol following the beginning of the study, where we developed a method to measure fetal blood CO levels.
Octyl-D-2-hydroxyglutarate was synthesized following the method reported by Xu et al. [ 12] with slight modifications (detailed protocol for D-2-hydroxyglutarate synthesis, see Additional file 1 and Additional file 2: Figure S1).
Also, the virtual correlation coefficients between different protocols are high (average 0.95), suggesting that slight differences in protocols based on T7 linear amplification mechanism do not affect the correlation of amplified samples to unamplified samples.
It's a touchy decision to deviate even the slightest from Olympic protocol.
(The palace, still traumatized by the abdication of her uncle, King Edward VIII, sixteen years earlier, sees the slightest aberration from protocol as a potential unravelling).
Suspensions of peripheral blood mononuclear cells (PBMC) from seven crossbred Large White × Landrace pigs (6 months old) were prepared from heparinised peripheral blood samples collected under aseptic conditions during bleeding at the abattoir, using slight modifications of protocols described previously [ 12].
He made one slight change in the protocol, in deference to ethical standards developed since 1963.
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