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Diesel degradation steps were followed as per the standard procedure with slight modifications, as discussed by Smita et al. [13].
Plastic degradation steps were followed as per the standard procedure with slight modifications, as discussed by Kathiresan [12].
This plan and the "Esquisse de Gouvernement provisoire" with slight modifications as transmitted by Miranda to Spanish America in 1808 are printed in Robertson, Miranda, pp520‑25.
Chemical modification reactions with anhydride reagents were carried out with slight modifications as described in Means and Feeney (Means and Feeney 1971) and Wong Wongg 2000).
Cytotoxicity induced by the drug assessed by lactate dehydrogenase (LDH) leakage into the culture medium was carried out with slight modifications as described in Fotakis and Timbrell 2006 [8].
The study followed the guidelines of the IHS with slight modifications, as the diagnosis CEH according to the criteria can only be given retrospectively after resolution of the symptoms [5].
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The activity of the PB-degrading enzyme in culture broth was determined as described previously (Kitamoto et al. [2011]) with a slight modification as follows: The supernatant (200 μL ) obtained by filtering the culture broth was added to 1.8 mL of 20 mM Tris HCl buffer solution (pH 6.8) containing emulsified PBSA in a glass test tube (10 mm internal diameter).
The concentration (C) of carotenoid in the sample was calculated using the equation given by Saito and Regier (1971) with a slight modification as follows: C ( mg / g lipids) = A 468 × volume of extract × dilution factor 0.2 × weight of sample ( g) where 0.2 is the A468 of 1 µg/ml standard astaxanthin.
DH was calculated following the method of Benjakul and Morrissey (1997) with a slight modification as follows: % DH = L / L max × 100 where L is the amount of free amino group in the product and Lmax is the total free amino group after acid hydrolysis (6 M HCl at 100 °C for 24 h).
Image analysis proceeded, with slight modification, as detailed elsewhere [55].
Assays were adapted with slight modification as described previously (Senapati et al, 2010).
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