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Protein content was measured using a standard BCA Assay (Thermo Scientific, Waltham, MA, USA) and lysates were treated according to manufacturer's instructions, with slight method alteration in the detergent removal step.
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Other investigators obtained similar results with the IOS, a slight different method of FOT from the method in the present study [ 5, 6].
It was described that the best efficiency device was obtained from a slight modified method of prewetting of the PbI2 film by dipping in 2-propanol for one second prior to being dipped in the MAI solution.
Evidently, in terms of controlling the probability of a Type I error, alternative methods would provide at best a slight improvement over method HC among the situations considered in the simulations simply because HC performs well.
With slight modifications, this method is used to produce spinel, rutile, and strontium titanate.
Slight variations in method have acted as a kind of digital signature, to help investigators trace who they are, our correspondent says.
After slight modification, the method also enabled baseline resolution of the most commonly encountered amphetamine derivatives, as well as cocaine and heroin, within 7 min.
AChE and BuChE inhibiting activities were measured by the slight modified spectrophotometric method of Ellman using a 96-well plate reader [36].
Thioflavin-S staining (Sigma) was performed as previously described [37], [38] with slight modifications of method.
The standardized pentaplex PCR assay designed to detect Shigella directly from stool was also tested using stool samples spiked with a known amount of Shigella based on slight modification of method described by Houng et al. [ 18].
It is unclear if this slight difference in methods between our study and the method proposed by Fujimura et al would result in significantly different concentrations, or significantly impact the decay of the solutions stored at various environmental conditions.
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