Moreover, most transcripts in this group showed slight alterations in expression level between the libraries.
In proliferating tissues and actively dividing cells, there is a slight increase in expression level (approximately twofold) [ 41, 42].
A slight reduction in expression level correspondence from r = 0.54 to r = 0.51 was also seen in MCF-10a.
By contrast, in Myc cells the Bcl-2 was not phosphorylated after PTX treatment and only a slight decrease in its expression level was observed (Figure 2D).
The results showed that expressions of Cyclin A, Cyclin D1, CDK2, and CDK4 were notably up-regulated and a slight increase in CyclinE expression level, while Rb, p27kip1 and p53 were down-regulated in CHD1L-transgenic MEFs compared with wildtype MEFs (Fig. 6C).
All other components of the ethylene signaling machinery showed no or only slight changes in the expression level.
While the presence of the constitutive promoter might directly account for the observed slight increase in background expression level, the decrease in gene induction levels might be explained by an insufficient concentration of M2-transactivator generated by the PGK-promoter.
Here, we found a slight induction of HIPK2 expression level in HIPK2+/+ cells after 8 h glu stv, which decreased in the subsequent time points, suggestive of HIPK2 activation, while siHIPK2 cells were negative (Supplementary Figure S2).
The data showed that there was a slight increase in the expression level of GR in alantolactone-treated cell lysates, further confirming that GSH depletion is not linked with its oxidation to GSSG).
Since N-CoR and SMRT are common corepressors for transcription factors, slight alteration of their expression level in certain tissues might result in significant transcriptional changes, leading to altered development of the mammary gland, even tumors.
